NeCEN is an open access facility for high resolution cryo-electron microscopy of biological samples. A state-of-the-art Titan Krios transmission electron microscope allows efficient and automated high resolution data collection in its two most broadly used forms, single particle analysis (SPA) and cryo-electron tomography (cryo-ET). These imaging techniques allow visualisation of biomolecular structures, such as proteins, macromolecular complexes, bacteria, and cell organelles at subnanometer resolution in close-to-native conditions. The facility is equipped with a BSL2 lab and microscope rooms.
The Titan Krios5 at NeCEN is equipped with a C-FEG and Falcon 4i Selectris. Extended range Aberration-Free Image Shift (AFIS) is possible, allowing SPA data collection speeds of up to 900 movies per hour.
The Titan Krios 5 is a dedicated instrument for energy-filtered TEM (EFTEM) and cryo-electron tomography. This microscope is equipped with a cold-FEG and Falcon4i Selectris. It is an instrument for both tomography and automated single particle acquisition.
NeCEN is open for applications from Dutch and international academia as well as industry. Access via the Instruct route is subsidized. Contact us for details.
